Nope, this skull is not Cleopatra’s half-sister

Scientists have demonstrated that an ancient human skull excavated from a tomb at Ephesos was not that of Arsinoë IV, half-sister to Cleopatra VII. Rather, it’s the skull of a young male between the ages of 11 and 14 from Italy or Sardinia, who may have suffered from one or more developmental disorders, according to a new paper published in the journal Scientific Reports. Arsinoë IV’s remains are thus still missing.

Arsinoë IV led quite an adventurous short life. She was either the third or fourth daughter of Ptolemy XII, who left the throne to Cleopatra and his son, Ptolemy XIII, to rule together. Ptolemy XIII didn’t care for this decision and dethroned Cleopatra in a civil war—until Julius Caesar intervened to enforce their father’s original plan of co-rulership. As for Arsinoë, Caesar returned Cyprus to Egyptian rule and named her and her youngest brother (Ptolemy XIV) co-rulers. This time it was Arsinoë who rebelled, taking command of the Egyptian army and declaring herself queen.

She was fairly successful at first in battling the Romans, conducting a siege against Alexandria and Cleopatra, until her disillusioned officers decided they’d had enough and secretly negotiated with Caesar to turn her over to him. Caesar agreed, and after a bit of public humiliation, he granted Arsinoë sanctuary in the temple of Artemis in Ephesos. She lived in relative peace for a few years, until Cleopatra and Mark Antony ordered her execution on the steps of the temple—a scandalous violation of the temple as a place of sanctuary. Historians disagree about Arsinoë’s age when she died: Estimates range from 22 to 27.

Archaeologists have been excavating the ancient city of Ephesos for more than a century. The Octagon was uncovered in 1904, and the burial chamber was opened in 1929. That’s where Joseph Keil found a skeleton in a sarcophagus filled with water, but for some reason, Keil only removed the cranium from the tomb before sealing it back up. He took the skull with him to Germany and declared it belonged to a likely female around 20 years old, although he provided no hard data to support that conclusion.

It was Hilke Thur of the Austrian Academy of Sciences who first speculated that the skull may have belonged to Arsinoë IV, despite the lack of an inscription (or even any grave goods) on the tomb where it was found. Old notes and photographs, as well as craniometry, served as the only evidence. The skull accompanied Keil to his new position at the University of Vienna, and there was one 1953 paper reporting on craniometric measurements, but after that the skull languished in relative obscurity. Archaeologists at the University of Graz rediscovered the skull in Vienna in 2022. The rest of the skeleton remained buried until the chamber was reopened and explored further in the 1980s and 1990s, but it was no longer in the sarcophagus.